Mothers are assessed using this method. Procedures are courtesy of VIVADENT.
 |
|
Chew the Parrafin wax to simulate salivation for 5 minutes. |
|
 |
|
Collect Saliva in measuring cup (saliva flow data is collected). |
|
 |
|
Open the agar surface vial. |
|
 |
|
Insert Sodium Bicarbonate tablet (Baking Soda) into the vial. This realeases CO2 into the vial for optimal anaerobic bacterial growth. |
|
 |
|
Peel off the protective film of both S. mutans agar surface (blue) and Lactobacillus agar surface (green). |
|
 |
|
Use the pippette to collect the saliva. |
|
 |
|
Saturate both sides of the agar surfaces without touching or disturbing it. |
|
 |
|
Use the measuring cup to collect the saliva that drips from the agar surface. |
|
 |
|
Label the vial with personal information and CRT test date. |
|
 |
|
Incubate the vial for 48 hours at 37 degrees celsius. |
|
 |
|
Compare bacteria colony growth with the chart. |
|
 |
|
Low levels of S. mutans. |
|
 |
|
Medium level of S. mutans. |
|
 |
|
High levels of S. mutans. |
|
 |
|
Low level of Lactobacillus. |
|
 |
|
Medium level of Lactobacillus. |
|
 |
|
High level of Lactobacillus. |
